![]() NAD(P)H:quinone oxidoreductase (NQO) is a ubiquitous flavoprotein enzyme which catalyzes the two-electron reduction/detoxification of quinones/quinones derivatives and it has two members - NQO1 (alias DT-diaphorase) which uses NADPH as a source of electrons, and NQO2 (NRH:quinone oxidoreductase 2) which employs dihydronicotinamide riboside (NRH) as electron donor. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Separated by Size-Wes, Sally Sue/Peggy Sue. ![]() In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In IHC-P, staining was observed in the cytoplasm of human breast cancer tisSue. In ICC/IF, cytoplasmic staining was observed in U2OS cells. In Western blot a band can be seen at approx. This NQO1 Antibody (A180) is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections and Flow Cytometry.
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